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High performance liquid chromatography (HPLC)

SAIL houses two high-performance liquid chromatographs for the separation and analysis of small molecules in solution.

Dionex UltiMate 3000 (Chemistry) Quaternary pump Auto-sampler injection Sample and column heaters Isocratic and gradient separations UV-Vis detector monitors up to 4 wavelengths simultaneously 3-D spectra	PerkinElmer Flexar (SAIL)    Binary pump Manual injection UV-Vis detector

Available columns

• Restek Ultra C18 5-micron 100 x 4.6 mm
• Restek Ultra C18 5-micron 150 x 4.6 mm
• Restek Ultra AQ C18 5-micron 150 x 4.6 mm
• Shodex KC-811 ion exchange column

Recent applications

• Determination of caffeine content of various beverages
• Identification of artificial dyes in foods
• Organic and analytical chemistry courses

Documents

Dionex UltiMate 3000 Standard Operating Procedure

PerkinElmer Flexar Standard Operating Procedure